FOX-04

Overview

FOX-04 is supplied under this catalogue designation for senescence research, and is generally catalogued elsewhere in the market as FOXO4-DRI, a D retro inverso peptide built to interfere with the interaction between the FOXO4 transcription factor and p53. The D retro inverso construction, in which D amino acids are assembled in reverse sequence order, retains the side chain topology of the parent sequence while making the molecule highly resistant to proteolysis, and that is the feature the senescence literature makes use of. Because catalogue naming is inconsistent across suppliers in this area, confirm the sequence held against the lot Certificate of Analysis before working to any published figures.

Areas of research interest

The points below describe where this material, or the compound class it belongs to, appears in the published scientific literature. They are provided to help researchers scope a study design. They are not claims about what this product does and no outcome of any kind is represented.

• Cellular senescence models and senescent cell burden assays
• FOXO4 and p53 protein interaction studies
• Comparative work between D retro inverso constructs and their L form parent sequences
• Proteolytic stability studies on retro inverso peptides

Specifications

Handling, reconstitution and storage

• Store sealed vials at -20 °C, protected from light. Short excursions to 2 to 8 °C in transit are expected; return vials to frozen storage on receipt.
• Reconstitute with bacteriostatic water or another diluent appropriate to your protocol. Add the diluent slowly against the inner wall of the vial and allow the cake to dissolve without agitation. Do not shake.
• After reconstitution, store at 2 to 8 °C and protect from light. Establish an in-use period from your own stability testing rather than from a supplier figure.
• Avoid repeated freeze-thaw cycles. Aliquot where a protocol calls for multiple withdrawals from a single vial.
• Use aseptic technique and standard personal protective equipment throughout. Allow vials to reach room temperature before opening to limit condensation.

Certificate of Analysis

Every lot is supplied with its own Certificate of Analysis. Identity, content and purity figures are those stated on that document for the specific lot supplied, and researchers should verify identity and suitability against their own analytical methods before the material is put into a study.

Research use only

This product is supplied strictly for laboratory research use only. It is not a drug, food, cosmetic or dietary supplement. It is not intended to diagnose, treat, cure or prevent any disease, and it is not intended for human or veterinary consumption or for administration to humans or animals in any form. It must be handled only by qualified researchers in an appropriate laboratory setting, and it is the purchaser who is responsible for ensuring that their intended use complies with all laws and institutional requirements that apply to them.